背景描述: WM266-4 是一種轉(zhuǎn)移性人黑色素瘤細(xì)胞系,具有小扁平間充質(zhì)形態(tài)。該細(xì)胞系來(lái)源于與細(xì)胞系 WM115、WM239A 和 WM165-1 相同的患者。WM115細(xì)胞系來(lái)源于原發(fā)腫瘤,WM266-4、WM165-1、WM239A來(lái)源于單個(gè)淋巴結(jié)轉(zhuǎn)移瘤。受試者(55 歲女性)顯示 VGP 伴 Clark III 級(jí)腫瘤,厚度為 2.24mm。
培養(yǎng)建議
生長(zhǎng)形態(tài): 小扁平間充質(zhì)
生長(zhǎng)特性: 貼壁
培養(yǎng)條件: DMEM+10%FBS+1%P/S
凍存條件: 90%FBS+10%DMSO
血清使用等級(jí): 優(yōu)級(jí)
傳代方式: 胰酶消化
倍增時(shí)間: 30h
傳代比例: 胰酶消化
換液頻率: 每周2~3次
生物安全等級(jí): 1
培養(yǎng)注意事項(xiàng):
其他信息
基因表達(dá): WM266-4 cell line is wild type for N-RAS, c-KIT, and CDK4 genes.
致瘤性: WM266-4 cells produce xenograft tumors when injected into immunocompromised mice.
突變信息: This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion.
人黑色素瘤細(xì)胞
Catalog NoCL0718
Product information
基因表達(dá): WM266-4 cell line is wild type for N-RAS, c-KIT, and CDK4 genes.
致瘤性: WM266-4 cells produce xenograft tumors when injected into immunocompromised mice.
突變信息: This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion.